Application of auxin-inducible degron technology to mouse oocyte activation with PLCζ
نویسندگان
چکیده
منابع مشابه
Human PLCζ exhibits superior fertilization potency over mouse PLCζ in triggering the Ca(2+) oscillations required for mammalian oocyte activation.
A sperm-specific phospholipase C-zeta (PLCζ) is believed to play an essential role in oocyte activation during mammalian fertilization. Sperm PLCζ has been shown to trigger a prolonged series of repetitive Ca(2+) transients or oscillations in oocytes that precede activation. This remarkable intracellular Ca(2+) signalling phenomenon is a distinctive characteristic observed during in vitro ferti...
متن کاملDiagnosis of genetic defects through parallel assessment of PLCζ and CAPZA3 in infertile men with history of failed oocyte activation
Objective(s): Phospholipase C ζ (PLCζ) is considered as a nominee for sperm associated oocyte activating factors and is located back-to-back with CAPZA3, an actin-capping protein controlling actin polymerization during spermiogenesis. They contain a common bidirectional promoter. The objective of this study was to identify individuals with parallel low expression of PLCζ and CAPZA3 mRNA, in hop...
متن کاملeffect of ultrasound on parthenogenic activation of mouse oocyte
background: artificial stimulation of mouse oocyte, in the absence of sperm contribution, can induce its parthenogenic activation of oocyte. ultrasound is one of the newest methods for artificial activation of mammal oocytes, and its successful utilization in pig oocyte activation has been recently reported. our objective was to assess the effect of ultrasound on mouse oocyte activation. materi...
متن کاملAn expanded tool kit for the auxin-inducible degron system in budding yeast
Fusion of inducible degradation signals, so-called degrons, to cellular proteins is an elegant method of controlling protein levels in vivo. Recently, a degron system relying on the plant hormone auxin has been described for use in yeast and vertebrate cells. We now report the construction of a series of vectors that significantly enhance the versatility of this auxin-inducible degron (AID) sys...
متن کاملdiagnosis of genetic defects through parallel assessment of plcζ and capza3 in infertile men with history of failed oocyte activation
objective(s): phospholipase c ζ (plcζ) is considered as a nominee for sperm associated oocyte activating factors and is located back-to-back with capza3, an actin-capping protein controlling actin polymerization during spermiogenesis. they contain a common bidirectional promoter. the objective of this study was to identify individuals with parallel low expression of plcζ and capza3 mrna, in hop...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 2018
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.2018-053